1548 Isabella of Portugal by Tiziano Vecelli ("Titian") (Prado)
An overall view of Titian's 1548 portrait of Isabella of Portugal wearing Iberian-style dress. She married Charles V. One of her sons, Felipe became Felipe II, King of Spain. Three of Felipe's four wives are subjects of Minialbums. The next four images are parts of this portrait.
Her Wikipedia article can be found here.