ca. 1623 Elizabeth, Queen of Bohemia by Michiel Jansz. van Miereveldt (National Portrait Gallery - London UK)
She married in 1613 and took up residence in Heidelburg.
The Wikipedia article for Michiel Jansz. van Miereveldt (also spelled van Mierevelt) is here.
From Wikimedia; linear flaws in the background fixed with Photoshop by gogm.